Anti-PCNA Mouse mAb

Antibody type:Loading control antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,IP,ICC,IHC

Purify method:Fractional precipitation

Species:Human,Mouse,Monkey,Rat,Hamster

Gene Name:pcna

Synonyms:Cyclin;DNA polymerase delta auxiliary protein;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating Cell Nuclear Antigen.

Gene Synonyms:Cyclin;DNA polymerase delta auxiliary protein;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating Cell Nuclear Antigen.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human PCNA protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2E1-G10-H10

Dilution:WB 1:1000,ICC/IHC 1:100，IHC 1:400

Mole Mass:36kDa

Location:Nucleus.

Concentration:

Sequence Similarity:Belongs to the PCNA family.

Gene Id:5111

SwissProt ID:P12004

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.Has to be loaded onto DNA in order to be able to stimulate APEX2.Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair:Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases,while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.