Anti-PKCa Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Rat,Monkey

Gene Name:PKCa

Synonyms:AAG6; PKCA; PRKACA; PKC-alpha

Gene Synonyms:AAG6;Aging associated gene 6;aPKC;KPCA_HUMAN;PKC alpha;PKC-A;PKC-alpha;PKCA;PRKACA;PRKCA;Protein Kinase C alpha;Protein kinase C alpha type.

Gene Full Name:

Gene Infomation:

Antigen:Synthetic peptide corresponding to human PKCa protein

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:PKCα mouse mAb recognizes endogenous levels of total PKCα protein.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3G11-G11-G11

Dilution:WB 1:1000

Mole Mass:80kDa

Location:Cytoplasm.

Concentration:4.97mg/ml

Sequence Similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily._x000D_Contains 1 AGC-kinase C-terminal domain._x000D_Contains 1 C2 domain._x000D_Contains 2 phorbol-ester/DAG-type zinc fingers._x000D_Contains 1 protein kinase domain.

Gene Id:5578

SwissProt ID:P17252

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:PKC alpha is an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.