Anti-PKM2 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IF

Purify method:Affinity purified

Species:Human,Mouse,Rat,Monkey

Gene Name:pkm2

Synonyms:PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

Gene Synonyms:CTHBP;Cytosolic thyroid hormone binding protein;Cytosolic thyroid hormone-binding protein;KPYM_HUMAN;MGC3932;OIP 3;OIP-3;OIP3;OPA interacting protein 3;Opa-interacting protein 3;p58;PK muscle type;PK, muscle type;PK2;PK3;PKM;PKM2;pykm;Pyruvate kinase 2/3;

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human PKM2 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of PKM2 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:1A7-G6-H6

Dilution:WB 1:1000,ICC 1:400

Mole Mass:60kDa

Location:Cytoplasm_x000D_Nucleus

Concentration:

Sequence Similarity:Belongs to the pyruvate kinase family.

Gene Id:5315

SwissProt ID:P14618

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Pyruvate kinase is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.