Anti-PKR Rabbit pAb
- 100ug (NP21357) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,ICC/IF,IP
Purify method:Affinity Chromatography
Species:Human
Gene Name:
Synonyms:eIF-2A protein kinase 2; Protein kinase RNA-activated; PKR; p68 kinase; P1/eIF-2A protein kinase;
Gene Synonyms:
Gene Full Name:
Gene Infomation:PKC is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
Antigen:A synthesized peptide derived from human PKR
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:PKR Antibody detects endogenous levels of PKR
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:10000~1:20000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
Mole Mass:68kDa
Location:Cell junction;
Concentration:
Sequence Similarity:Contains 1 protein kinase domain.
Gene Id:
SwissProt ID:P19525
Unigene:
Nucleotide Accession:
Tissue specificity:PKR Antibody detects endogenous levels of PKR
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:PKR a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.