Anti-PLA2G4A (Center) Rabbit pAb

Antibody type:Flowcytometry

Label:Unconjugated

Modification:

Isotype:Rabbit Ig

Host:Rabbit

Application:WB,IHC-P,IF,FC

Purify method:Affinity purified

Species:Human

Gene Name:pla2g4a

Synonyms:Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4A, CPLA2, PLA2G4

Gene Synonyms:

Gene Full Name:

Gene Infomation:PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.

Antigen:This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the Central region of human PLA2G4A.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:HUMAN

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:RB21320

Dilution:WB::1:1000ICC::1:10~50IHC::1:10~50FCM::1:10~50

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Storage:

Buffer condition:Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Background:

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Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.