Anti-POU5F1/OCT4 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Mouse

Gene Name:pou5f1

Synonyms:Oct3;Oct4;Otf3;Otf4;NF-A3;Oct-3;Oct-4;Otf-3;Otf-4;Otf3g;Oct3/4;Oct-3/4;Otf3-rs7.

Gene Synonyms:MGC22487;Oct 3;Oct 4;Oct-3;Oct-4;OCT3;Oct4;Octamer binding protein 3;Octamer binding protein 4;Octamer binding transcription factor 3;Octamer-binding protein 3;Octamer-binding protein 4;Octamer-binding transcription factor 3;OTF 3;OTF 4;OTF-3;OTF3;OTF4; P

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Mouse

Reaction:This antibody detects endogenous levels of POU5F1/OCT4 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3F5-F9-E5

Dilution:WB 1:1000,Other 1:1000

Mole Mass:45kDa

Location:Nucleus.

Concentration:

Sequence Similarity:Belongs to the POU transcription factor family. Class-5 subfamily.Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.

Gene Id:18999

SwissProt ID:P20263

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol.

Background:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.