Anti-PP2A α and β Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:WB,IP

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:ppp2cb

Synonyms:PP2CB;PP2Abeta;PP2A alpha and beta.

Gene Synonyms:PP2A beta;PP2A-beta;PP2AB_HUMAN;PP2Abeta;PP2CB;Ppp2cb;Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform;Protein phosphatase 2 catalytic subunit beta isozyme;Protein phosphatase 2, catalytic subunit, beta isoform;Protein phosphatase 2A c

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human full length PP2A beta protein expressed in E.coli

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of PP2A alpha and PP2A beta.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2G2-H6-G11-E8

Dilution:WB 1:2000

Mole Mass:36kDa

Location:Cytoplasm.Nucleus.Chromosome ? centromere.Cytoplasm ? cytoskeleton ? spindle pole.Note: In prometaphase cells,but not in anaphase cells, localizes at centromeres.During mitosis,also found at spindle poles.

Concentration:

Sequence Similarity:Belongs to the PPP phosphatase family.PP-1 subfamily.

Gene Id:5516

SwissProt ID:P62714

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol.

Background:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2,mitogen-stimulated S6 kinase,and MAP-2 kinase.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.