Anti-PRAS40 Rabbit pAb
- 100ug (NP20536) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IP
Purify method:Affinity Chromatography
Species:Human,Mouse,Rat
Gene Name:
Synonyms:40 kDa proline rich AKT substrate; AKT1 S1; Lobe; PRAS40; Proline rich akt substrate; Proline rich Akt substrate 40 kDa;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich prote
Antigen:A synthesized peptide derived from human PRAS40
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:PRAS40 Antibody detects endogenous levels of total PRAS40
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:500~1:2000 IP 1:50
Mole Mass:40kDa
Location:Cytoplasm > Cytosol;
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:Q96B36
Unigene:
Nucleotide Accession:
Tissue specificity:PRAS40 Antibody detects endogenous levels of total PRAS40
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.