Anti-PRMT1 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human

Gene Name:prmt1

Synonyms:ANM1;HCP1;IR1B4;HRMT1L2.

Gene Synonyms:ANM 1;ANM1;ANM1_HUMAN;complete cds;HCP 1;HCP1;Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2;Heterogeneous nuclear ribonucleoproteins methyltransferase like 2;Heterogeneous nuclear ribonucleoproteins methyltransferase like2;Histone-arg

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human PRMT1 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of PRMT1 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4E9-H9-H12

Dilution:WB 1:1000

Mole Mass:42kDa

Location:Nucleus.Nucleus ? nucleoplasm by similarity.Cytoplasm ? cytosol by similarity.Note: Mostly found in the cytoplasm.Colocalizes with CHTOP within the nucleus.Low levels detected also in the chromatin fraction by similarity.

Concentration:

Sequence Similarity:Belongs to the class I-like SAM-binding methyltransferase superfamily.Protein arginine N-methyltransferase family.Contains 1 SAM-dependent Mtase PRMT-type domain.

Gene Id:3276

SwissProt ID:Q99873

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.