Anti-PRMT5Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5; Skb1Hs Methyltransferase;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Unknown. Cellular response protein to viral infection.

Antigen:A synthesized peptide derived from human PRMT5

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:PRMT5 Antibody detects endogenous levels of total PRMT5

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

Mole Mass:73kDa

Location:Golgi apparatus > cis-Golgi network membrane > Single-pass type II membrane protein;

Concentration:

Sequence Similarity:Belongs to the GOLM1/CASC4 family.

Gene Id:

SwissProt ID:O14744

Unigene:

Nucleotide Accession:

Tissue specificity:PRMT5 Antibody detects endogenous levels of total PRMT5

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p. An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.