Anti-Phospho-AMPK alpha 1 (S496) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF,IP

Purify method:Affinity Chromatography

Species:Human

Gene Name:

Synonyms:5'-AMP-activated protein kinase catalytic subunit alpha-1; AAPK1; AMP-activate kinase alpha 1 subunit; AMP-activated protein kinase; AMPK; AMPK alpha 1; AMPK subunit alpha-1; PRKAA 1; ACACA kinase;

Gene Synonyms:

Gene Full Name:

Gene Infomation:GSK3B a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation.

Antigen:A synthesized peptide derived from human AMPK alpha 1

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-AMPK alpha 1 (S496) Antibody detects endogenous levels of Phospho-AMPK alpha 1 (S496)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000 ICC/IF 1:50~1:200 IP 1:50

Mole Mass:64kDa

Location:Cell membrane;

Concentration:

Sequence Similarity:Contains 1 protein kinase domain.

Gene Id:

SwissProt ID:Q13131

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-AMPK alpha 1 (S496) Antibody detects endogenous levels of Phospho-AMPK alpha 1 (S496)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.