Call Now

Anti-Phospho-AMPK alpha 2 (S491) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:5'-AMP-activated protein kinase catalytic subunit alpha-2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMPK alpha 2 chain; AMPK subunit alpha-2; AMPK2; AMPKalpha2; PRKAA2;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways.

Antigen:A synthesized peptide derived from human AMPK alpha 2

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-AMPK alpha 2 (S491) Antibody detects endogenous levels of Phospho-AMPK alpha 2 (S491)

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000

Mole Mass:62kDa

Location:Cell membrane > Peripheral membrane protein;

Concentration:

Sequence Similarity:Contains 1 protein kinase domain.

Gene Id:

SwissProt ID:P54646

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-AMPK alpha 2 (S491) Antibody detects endogenous levels of Phospho-AMPK alpha 2 (S491)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.