Anti-Phospho-Akt(Ser473) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:AKT; AKT1 kinase; C-AKT; PKB; PKB-alpha; Protein kinase B; RAC; RAC-PK-alpha;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Belongs to the annexin family.Contains 4 annexin repeats.

Antigen:A synthesized peptide derived from human Phospho-Akt(Ser473)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-Akt(Ser473) Antibody detects endogenous levels of total Phospho-Akt(Ser473)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:100~1:500

Mole Mass:60kDa

Location:

Concentration:

Sequence Similarity:Belongs to the annexin family.

Gene Id:

SwissProt ID:P31749

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-Akt(Ser473) Antibody detects endogenous levels of total Phospho-Akt(Ser473)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:An AGC kinase that plays a critical role in controlling the balance between survival and AP0ptosis. Phosphorylated and activated by PDK1 in the PI3 kinase pathway. Mediates survival signals downstream of PI3 kinase and several growth factor receptors by phosphorylating AP0pototic proteins. First found in a mouse transforming retrovirus.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.