Anti-Phospho-Akt (Thr308) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Phosphoralated

Isotype:Rabbit IgG

Host:Rabbit

Application:WB

Purify method:Affinity purified

Species:Mouse

Gene Name:akt

Synonyms:AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Gene Synonyms:AKT1;AKT1_HUMAN;AKT2;AKT3;PKB alpha;PKB;PKB beta;PKBalpha;PRKBA;PRKBB;PRKBG;Protein kinase B;Protein kinase B beta;Protein kinase B gamma;Proto-oncogene c-Akt;RAC alpha;RAC;RAC gamma;RAC PK alpha;RAC PK beta;RAC PK gamma;RAC-alpha serine/threonine-protein

Gene Full Name:

Gene Infomation:

Antigen:DGATMKp-TFCGTPE

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500

Mole Mass:60kDa

Location:Cytoplasm_x000D_Nucleus_x000D_Cell membrane

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily._x000D_Contains 1 AGC-kinase C-terminal domain._x000D_Contains 1 PH domain._x000D_Contains 1 protein kinase domain.

Gene Id:207

SwissProt ID:P31749

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:10mM PBS with 0.02% sodium azide 50% glycerol.

Background:Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.