Anti-Phospho-B Raf (T401) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF,IP

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; BRAF1; RAFB1; RMIL; RMIL serine/threonine-protein kinase; c-RMIL; kinase B-Raf;

Gene Synonyms:

Gene Full Name:

Gene Infomation:The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Antigen:A synthesized peptide derived from human Phospho-B Raf (T401)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-B Raf (T401) Antibody detects endogenous levels of total Phospho-B Raf (T401)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:30

Mole Mass:85kDa

Location:

Concentration:

Sequence Similarity:Belongs to the intermediate filament family.

Gene Id:

SwissProt ID:P15056

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-B Raf (T401) Antibody detects endogenous levels of total Phospho-B Raf (T401)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.