Anti-Phospho-Cdc6 (S54) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF

Purify method:Affinity Chromatography

Species:Human

Gene Name:

Synonyms:CDC6; CDC18L; CDC6-related protein; Cdc18-related protein; HsCDC6; HsCDC18; p62(cdc6);

Gene Synonyms:

Gene Full Name:

Gene Infomation:Mitofusins are mitochondrial transmembrane GTPases that function to regulate mitochondrial fusion, a process that occurs in concert with mitochondrial division and is necessary for the maintenance of structural and genetic mitochondrial integrity. Two mitofusins have been described in mammals, mitofusin-1 and -2, which share 60% amino acid identity and appear to function coordinately to regulate mitochondrial fusion. Mitochondrial fusion is widely recognized as important for normal cell growth and development, and may have evolved as a mechanism to offset the deleterious effects of mtDNA mutations. Null mutations in either mitofusin are embryonic lethal in mice, whereas conditional knockout studies have shown that combined deletion of mitofusin-1 and mitofusin-2 in skeletal muscle results in severe mitochondrial dysfunction.

Antigen:A synthesized peptide derived from human Phospho-Cdc6 (S54)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-Cdc6 (S54) Antibody detects endogenous levels of total Phospho-Cdc6 (S54)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:1000 ICC/IF 1:50~1:200

Mole Mass:63kDa

Location:Cytoplasm;

Concentration:

Sequence Similarity:Contains 1 dynamin-type G (guanine nucleotide-binding) domain.

Gene Id:

SwissProt ID:Q99741

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-Cdc6 (S54) Antibody detects endogenous levels of total Phospho-Cdc6 (S54)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.Cdc6 has recently been shown to play an important role in the intra-S-phase p21 Waf1/Cip1-dependent DNA damage response. Both cdc6 and CDT1 are degraded by the ubiquitin proteasome pathway in response to DNA damage associated with re-replication.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.