Anti-Phospho-Histone H3 (Ser10) Rabbit pAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Phosphoralated
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IF
Purify method:Affinity purified
Species:Human
Gene Name:Histone H3
Synonyms:H3/A; H3FA
Gene Synonyms:Histone H3/f; Histone H3; Histone H3/d; Histone H3/b; Histone H3/c; Histone H3/a; Histone H3/l; Histone H3/j; Histone H3/k; Histone H3/h; H3/A; H3FA; Histone H3/i.
Gene Full Name:
Gene Infomation:
Antigen:Human Phospho-Histone H3(Ser10) synthetic peptide conjugated to KLH.
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:This antibody detects endogenous levels of Phospho-Histone H3(Ser10) and does not cross-react with related proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:1000,ICC 1:200
Mole Mass:17kDa
Location:Nucleus Chromosome
Concentration:
Sequence Similarity:Belongs to the histone H3 family.
Gene Id:8350
SwissProt ID:P68431
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.