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Anti-Phospho-IKB alpha (S32) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IP

Purify method:Affinity Chromatography

Species:Human

Gene Name:

Synonyms:I-kappa-B-alpha; IKBA; IkappaBalpha; MAD3; NFKBI; NFKBIA; RL/IF-1;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

Antigen:A synthesized peptide derived from human Phospho-IKB alpha (S32)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-IKB alpha (S32) Antibody detects endogenous levels of total Phospho-IKB alpha (S32)

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IP 1:50

Mole Mass:39kDa

Location:Cell membrane;

Concentration:

Sequence Similarity:Belongs to the heat shock protein 70 family.

Gene Id:

SwissProt ID:P25963

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-IKB alpha (S32) Antibody detects endogenous levels of total Phospho-IKB alpha (S32)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.