Anti-Phospho-PKC alpha (T638) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:EC 2.7.11.13; KPCA; PKC III; PKC-A; PKC-III; PKC-alpha; PRKACA; PRKCA; Protein kinase C; alpha type; kinase PKC-alpha;

Gene Synonyms:

Gene Full Name:

Gene Infomation:CDK2 is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. This protein can be regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B).

Antigen:A synthesized peptide derived from human Phospho-PKC alpha (T638)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-PKC alpha (T638) Antibody detects endogenous levels of total Phospho-PKC alpha (T638)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

Mole Mass:80kDa

Location:Endosome;

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Gene Id:

SwissProt ID:P17252

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-PKC alpha (T638) Antibody detects endogenous levels of total Phospho-PKC alpha (T638)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:PKC alpha is an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.