Anti-Phospho-PKR (T446) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,IP,ChIP

Purify method:Affinity Chromatography

Species:Human

Gene Name:

Synonyms:E2AK2;E2AK2; EIF2AK1; EIF2AK2; MGC126524; PKR p68 kinase; PKR; PRKR;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Histone methyltransferase. Methylates Lys-79 of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Antigen:A synthesized peptide derived from human Phospho-PKR (T446)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-PKR (T446) Antibody detects endogenous levels of total Phospho-PKR (T446)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50

Mole Mass:62kDa

Location:Nucleus;

Concentration:

Sequence Similarity:Contains 1 DOT1 domain.

Gene Id:

SwissProt ID:P19525

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-PKR (T446) Antibody detects endogenous levels of total Phospho-PKR (T446)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:PKR a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.