Anti-Phospho-RPA2 (T21) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IP

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:REPA2; RF-A; RP-A; RPA2; RPA32; replication factor-A protein 2; replication protein A 32 kDa subunit; replication protein A2;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Antigen:A synthesized peptide derived from human Phospho-RPA2 (T21)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-RPA2 (T21) Antibody detects endogenous levels of Phospho-RPA2 (T21)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:5000~1:10000 IP 1:20

Mole Mass:32kDa

Location:Cytoplasm;

Concentration:

Sequence Similarity:Belongs to the intermediate filament family.

Gene Id:

SwissProt ID:P15927

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-RPA2 (T21) Antibody detects endogenous levels of Phospho-RPA2 (T21)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.