Anti-Phospho-Raf1 (S621) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:C-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1;

Gene Synonyms:

Gene Full Name:

Gene Infomation:TK is a cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Antigen:A synthesized peptide derived from human Phospho-Raf1 (S621)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-Raf1 (S621) Antibody detects endogenous levels of total Phospho-Raf1 (S621)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000

Mole Mass:73kDa

Location:Cytoplasm;

Concentration:

Sequence Similarity:Belongs to the thymidine kinase family.

Gene Id:

SwissProt ID:P04049

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-Raf1 (S621) Antibody detects endogenous levels of total Phospho-Raf1 (S621)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.