Anti-Phospho-S6K1 (T421 + S424)Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IP

Purify method:Affinity Chromatography

Species:Human,Rat

Gene Name:

Synonyms:EC 2.7.11.1, KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, Ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K, kinase p70S6K, p70-S6K

Gene Synonyms:

Gene Full Name:

Gene Infomation:Products of the highly conserved Wnt gene family, including Wnt-1 through Wnt-10, play key roles in regulating cellular growth and differentiation. Wnt-1 is a cysteine-rich, secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1, which is essential for normal development of the embryonic nervous system, contributes to hyperplasia and tumorigenic progression when improperly expressed in mammary tissue. Wnt-3 is also involved in tumorigenesis and Wnt-2 and Wnt-4 may be associated with abnormal proliferation in human breast tissue. Wnt-1, Wnt-3 and Wnt-10b have been implicated along with FGF-3 in the development of mouse mammary tumor virus induced mouse mammary carcinomas. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somatic tissue.

Antigen:A synthesized peptide derived from human Phospho-S6K1 (T421 + S424)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Phospho-S6K1 (T421 + S424) Antibodydetects endogenous levels of total Phospho-S6K1 (T421 + S424)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IP 1:30

Mole Mass:70kDa

Location:Secreted > Extracellular space > Extracellular matrix;

Concentration:

Sequence Similarity:Belongs to the Wnt family.

Gene Id:

SwissProt ID:P23443

Unigene:

Nucleotide Accession:

Tissue specificity:Phospho-S6K1 (T421 + S424) Antibodydetects endogenous levels of total Phospho-S6K1 (T421 + S424)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.