Anti-Placental alkaline phosphatase (PLAP) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Phosphoralated

Isotype:IgG1

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human

Gene Name:alpp

Synonyms:ALPP; ALP; PALP; PLAP; PLAP-1

Gene Synonyms:Alkaline phosphatase; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; ALP; Alp1; ALPP; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; PALP; Placental alkaline phosphata

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant Placental alkaline phosphatase (PLAP) protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Placental alkaline phosphatase (PLAP) and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:8A11-C11-C9

Dilution:WB 1:1000

Mole Mass:70kDa

Location:Cell membrane; Lipid-anchor > GPI-anchor

Concentration:

Sequence Similarity:Belongs to the alkaline phosphatase family.

Gene Id:250

SwissProt ID:P05187

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Mammalian alkaline phosphatases (APs) are highly conserved zinc-containing allosteric enzymes that are able to hydrolyze and transphosphorylate a wide range of compounds. There are four known human alkaline phosphatase isozymes: TNAP (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPP2 (germ cell), and ALPI (intestinal) . Placental alkaline phosphatase (ALPP) is bound to the plasma membrane via a glycosyl-phosphatidylinositol (GPI) anchor. It is expressed primarily in the placenta and may be involved in transplacental IgG transport. ALPP has been found to be overexpressed on the surface of several different types of solid tumor cells and elevated serum concentrations of ALPP and ALPP-like enzymes has been found to be associated with ovarian, cervical, and testicular cancer.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.