Anti-Protein Phosphatase 4C Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Phosphoralated

Isotype:IgG2a

Host:Mouse

Application:WB,IHC,ICC

Purify method:Affinity purified

Species:Human

Gene Name:ppp4c

Synonyms:PP4; PPX; PP4C; PPH3; PPP4

Gene Synonyms:PP X;PP-X;PP4;PP4C;PP4C;PP4C_HUMAN;PPH3;PPP4;ppp4c;PPX;protein phosphatase 4 (formerly X), catalytic subunit;Protein phosphatase 4 catalytic subunit;Protein phosphatase X; Protein phosphatase X;protein phosphatase X, catalytic subunit;Serine/threonine pro

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Protein Phosphatase 4C protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Protein Phosphatase 4C and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2F11-D10-G4

Dilution:WB 1:200,ICC 1:200

Mole Mass:34kDa

Location:Cytoplasm. Nucleus

Concentration:

Sequence Similarity:Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily

Gene Id:5531

SwissProt ID:P60510

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.