Anti-RAD9A Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,IP

Purify method:Affinity purified

Species:Human,Mouse,Rat,Monkey

Gene Name:rad9a

Synonyms:RAD9

Gene Synonyms:Cell cycle checkpoint control protein;Cell cycle checkpoint control protein RAD9A;DNA repair exonuclease rad9 homolog A;hRAD 9;hRAD9;Rad 9;RAD 9A;RAD9 (S pombe) homolog;RAD9 homolog A;RAD9 homolog;RAD9A;RAD9A_HUMAN.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human RAD9A protein fragments expressed in E.coli

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of RAD9A and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3A3-A7-F8

Dilution:WB 1:500

Mole Mass:55kDa

Location:Nucleus

Concentration:

Sequence Similarity:Belongs to the rad9 family.

Gene Id:5883

SwissProt ID:Q99638

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in PBS(pH 7.4)containing with 0.02% sodium azide 50% glycerol.

Background:This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.