Anti-RAP80 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF

Purify method:Affinity Chromatography

Species:Human

Gene Name:

Synonyms:BRCA1-A complex subunit RAP80; RAP80; Receptor associated protein 80; RIP110; Rxrip110; UIMC1; X2HRIP110;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Ubiquitin-binding protein that specifically recognizes and binds Lys-63-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding Lys-63-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRC

Antigen:A synthesized peptide derived from human RAP80

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:RAP80 Antibody detects endogenous levels of total RAP80

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200

Mole Mass:80kDa

Location:Nucleus;

Concentration:

Sequence Similarity:Contains 2 UIM (ubiquitin-interacting motif) domains.

Gene Id:

SwissProt ID:Q96RL1

Unigene:

Nucleotide Accession:

Tissue specificity:RAP80 Antibodydetects endogenous levels of total RAP80

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.