Anti-RUNX1/RUNX2/RUNX3Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:RUNX1; AML1; RUNX2; OSF2; RUNX3; CBFA3;

Gene Synonyms:

Gene Full Name:

Gene Infomation:AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors. It is required for normal development of all hematopoietic lineages. AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. RUNX2 is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes including osteopontin, bone sialoprotein, and osteocalcin via binding to the core site of the enhancers or promoters.RUNX3/AML2 is a member of the Runt family of transcription factors. RUNX3 is important for the suppression of cell proliferation in the gastric epithelium, neurogenesis of the dorsal root ganglia, and T cell differentiation.

Antigen:A synthesized peptide derived from human RUNX1/RUNX2/RUNX3

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:RUNX1/RUNX2/RUNX3 Antibody detects endogenous levels of total RUNX1/RUNX2/RUNX3

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

Mole Mass:51kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:Q01196Q13761Q13950

Unigene:

Nucleotide Accession:

Tissue specificity:RUNX1/RUNX2/RUNX3 Antibody detects endogenous levels of total RUNX1/RUNX2/RUNX3

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:RUNX2 regulates the transcription of various genes including osteopontin, bone sialoprotein, and osteocalcin via binding to the core site of the enhancers or promoters.RUNX3/AML2 is a member of the Runt family of transcription factors. RUNX3 is important for the suppression of cell proliferation in the gastric epithelium, neurogenesis of the dorsal root ganglia, and T cell differentiation.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.