Anti-Raf-1 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IHC,ELISA

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:RAF1

Synonyms:RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Gene Synonyms:NS5; CRAF; Raf-1; c-Raf; CMD1NN

Gene Full Name:

Gene Infomation:This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Antigen:Synthesized peptide derived from human Raf-1 around the non-phosphorylation site of S289.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB::1/500-1/2000IHC::1/100-1/300

Mole Mass:75

Location:Nucleus;

Concentration:

Sequence Similarity:Contains 1 RBD (Ras-binding) domain.

Gene Id:5894

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:Raf-1 Polyclonal Antibody detects endogenous levels of Raf-1 protein.

Storage:

Buffer condition:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.