Anti-Renilla Luciferase Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:Renilla-type luciferase; Renilla luciferin 2 monooxygenase;

Gene Synonyms:

Gene Full Name:

Gene Infomation:FLI1 Sequence-specific transcriptional activator. Recognizes the DNA sequence 5-C[CA]GGAAGT-3. Belongs to the ETS family. Can form homodimers or heterodimers with ETV6/TEL1. 2 isoforms of the human protein are produced by alternative splicing.

Antigen:A synthesized peptide derived from Renilla Luciferase

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Renilla Luciferase Antibody detects endogenous levels of total Renilla Luciferase

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 ICC/IF 1:50~1:200

Mole Mass:35kDa

Location:Nucleus;

Concentration:

Sequence Similarity:Contains 1 PNT (pointed) domain.

Gene Id:

SwissProt ID:P27652

Unigene:

Nucleotide Accession:

Tissue specificity:Renilla Luciferase Antibody detects endogenous levels of total Renilla Luciferase

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + light. The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.