Anti-SH3GL2 Rabbit pAb

100ug (NP32916) Please inquiry

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IF

Purify method:Affinity purified

Species:Human,Mouse

Gene Name:SH3GL2

Synonyms:CNSA2; SH3P4; EEN-B1; SH3D2A

Gene Synonyms:

Gene Full Name:SH3 domain containing GRB2 like 2, endophilin A1

Gene Infomation:

Antigen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SH3GL2 (NP_003017.1).

Antigen Synonyms:Endophilin-A1

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB1:500 -1:2000; IF1:50 -1:200(Optimal dilutions should be determined by the end user)

Mole Mass:38kDa, 40kDa

Location:Cytoplasm, Endosome, Membrane

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:Q99962

Unigene:6456

Nucleotide Accession:

Tissue specificity:Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.Gene expression databases

Storage:Store at -20°C. Avoid freeze / thaw cycles.

Buffer condition:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Background:

Molar Function:Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.