Anti-SMAD4 (Phospho-Thr277) Rabbit pAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:
Isotype:Rabbit Ig
Host:Rabbit
Application:IF,DB,IHC-P
Purify method:Affinity purified
Species:Human
Gene Name:smad4
Synonyms:Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4
Gene Synonyms:
Gene Full Name:
Gene Infomation:Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily,SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
Antigen:This SMAD4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4.
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:HUMAN
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:RB07971
Dilution:ICC::1:10~50IHC::1:10~50Other::DB:1:500
Mole Mass:
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Sequence Similarity:
Gene Id:
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Unigene:
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Tissue specificity:
Storage:
Buffer condition:Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background:
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.