Anti-SMAD5(C-term) Mouse mAb

100ug (NP10060) Please inquiry

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,ICC,FC

Purify method:Affinity purified

Species:Human

Gene Name:smad5

Synonyms:DWFC; JV5-1; MADH5

Gene Synonyms:DKFZp781C1895;DKFZp781O1323;Dwfc;hSmad 5;hSmad5;JV5 1;JV5-1;MAD homolog 5;MAD mothers against decapentaplegic homolog 5;MAD, mothers against decapentaplegic homolog 5;MADH 5;MADH5;Mothers against decapentaplegic homolog 5;Mothers against DPP homolog 5;Mus

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of SMAD5 (C-terminus) and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4B10-B10-B6

Dilution:WB 1:1000,ICC 1:75,FCM 1:100

Mole Mass:60kDa

Location:Cytoplasm. Nucleus

Concentration:

Sequence Similarity:Belongs to the dwarfin/SMAD family._x000D_Contains 1 MH1 (MAD homology 1) domain._x000D_Contains 1 MH2 (MAD homology 2) domain

Gene Id:4090

SwissProt ID:Q99717

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol

Background:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Molar Function:

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.