Anti-SOX9 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:CMD1; Sox9; SRA1; SRXX2; SRXY10; SRY (sex determining region Y) box 9; Transcription factor SOX 9;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Midkine, or MK, is a heparin-binding molecule involved in the regulation of growth and differentiation during embryogenesis. MK expression is tightly regulated during embryonic development by steroid receptors of the retinoic acid superfamily. The mature human MK protein is 118 amino acids in length and contains five intrachain disulfide bonds. MK is a non-glycosylated protein that shows greater than 87% identity between human and mouse. The carboxy-terminus of MK contains the principle heparin-binding site and the molecule’s neurite-promoting sequences; both the amino- and carboxy-terminal sequences are required for the molecule’s neurotrophic properties. An association between overexpression of MK and colon adenocarcinoma has been shown in families suffering from familial polyposis. In addition, MK functions to enhance the activity of plasminogen activator (PA).

Antigen:A synthesized peptide derived from human SOX9

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:SOX9 Antibody detects endogenous levels of SOX9

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000 IHC 1:100~1:500 ICC/IF 1:50~1:200 IP 1:50 FC 1:100

Mole Mass:70kDa

Location:Secreted;

Concentration:

Sequence Similarity:Belongs to the pleiotrophin family.

Gene Id:

SwissProt ID:P48436

Unigene:

Nucleotide Accession:

Tissue specificity:SOX9 Antibody detects endogenous levels of SOX9

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification (2,3). Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.