Anti-STAT1 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:WB,IP

Purify method:Affinity purified

Species:Human,Monkey

Gene Name:stat1

Synonyms:CANDF7;ISGF-3;STAT91.

Gene Synonyms:Signal transducer and activator of transcription 1 91kD;DKFZp686B04100;ISGF 3;ISGF-3;OTTHUMP00000163552;OTTHUMP00000165046; OTTHUMP00000165047;OTTHUMP00000205845;Signal transducer and activator of transcription 1 91kDa;Signal transducer and activator of t

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human STAT1 protein fragments expressed in E.coli

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of total STAT1 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:6C2-F7-D12

Dilution:WB 1:1000

Mole Mass:91kDa

Location:Cytoplasm.Nucleus.Note: Translocated into the nucleus upon tyrosine phosphorylation and dimerization,in response to IFN-gamma and signaling by activated FGFR1,FGFR2,FGFR3 or FGFR4.

Concentration:

Sequence Similarity:Belongs to the transcription factor STAT family.Contains 1 SH2 domain.

Gene Id:6772

SwissProt ID:P42224

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.

Background:Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs),cytokine KITLG/SCF and other cytokines and other growth factors.Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors,signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor,that enters the nucleus.ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG),which drive the cell in an antiviral state.In response to type II IFN (IFN-gamma),STAT1 is tyrosine- and serine-phosphorylated.It then forms a homodimer termed IFN-gamma-activated factor (GAF),migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes,inducing a cellular antiviral state.Becomes activated in response to KITLG/SCF and KIT signaling.May mediate cellular responses to activated FGFR1,FGFR2,FGFR3 and FGFR4.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.