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Anti-SUMO-2/3 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human

Gene Name:SUMO2

Synonyms:HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2; SMT3A; Smt3B; SMT3H1; SUMO-3

Gene Synonyms:HSMT3 ;MGC117191 ;OTTHUMP00000115275 ;OTTHUMP00000115276 ;OTTHUMP00000115277 ;Sentrin 2 ;Small ubiquitin like modifier 2 ;Small ubiquitin related modifier 2 ;small ubiquitin-like modifier 3 ;small ubiquitin-related modifier 3 ;SMT3 homolog 1 ;SMT3 homolog 2 ;SMT3 suppressor of mif two 3 homolog 1 ;SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) ;SMT3 suppressor of mif two 3 homolog 2 ;SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) ;SMT3 suppressor of mif two 3 homolog 3 ;SMT3A ;SMT3B ;SMT3H1 ;SMT3H2 ;Sumo2 ;Sumo3 ;Ubiquitin like protein SMT3A ;Ubiquitin like protein SMT3B.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human SUMO-2/3 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:SUMO-2/3 Mouse mAb detects endogenous levels of SUMO-2/3. It does not cross-react with recombinant SUMO-1.

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3F7-C2-F8

Dilution:WB 1:1000

Mole Mass:

Location:Cytoplasmic (SUMO3) and Nuclear (SUMO2)

Concentration:0.29mg/ml

Sequence Similarity:

Gene Id:6613/6612

SwissProt ID:P61956P55854

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.