Anti-SUMO1 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IF

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:sumo1

Synonyms:DAP-1; GMP1; OFC10; PIC1; SENP2

Gene Synonyms:

Gene Full Name:

Gene Infomation:Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1, -2 or -3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IκB-α to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease (8-11).

Antigen:Recombinant Protein

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:12

Location:Nucleus membrane. Nucleus speckle. Cytoplasm.

Concentration:

Sequence Similarity:

Gene Id:7341

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:The antibody detects endogenous level of total SUMO1 protein.

Storage:

Buffer condition:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.