Anti-SUMO4 Rabbit pAb
- 100ug (NP21090) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,ICC/IF,IP,FC
Purify method:Affinity Chromatography
Species:Human,Mouse,Rat
Gene Name:
Synonyms:IDDM5; SMT3H4; SUMO-4; dJ281H8.4;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family. The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process analogous to ubiquitination. NEDD8 is first synthesized in a precursor form with a carboxy-terminal extension peptide that is removed by either the UCH-L3 or NEDP1/DEN1 hydrolase protein to yield a mature NEDD8 protein. Mature NEDD8 is then covalently linked to target proteins via the carboxy-terminal glycine residue in a reaction catalyzed by the APP-BP1/Uba3 heterodimer complex and Ubc12 as the E1- and E2-like enzymes, respectively.
Antigen:A synthesized peptide derived from human SUMO4
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:SUMO4 Antibody detects endogenous levels of total SUMO4
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Mole Mass:15kDa
Location:Nucleus;
Concentration:
Sequence Similarity:Belongs to the ubiquitin family.
Gene Id:
SwissProt ID:Q6EEV6
Unigene:
Nucleotide Accession:
Tissue specificity:SUMO4 Antibody detects endogenous levels of total SUMO4
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.