Anti-Smad2(Phospho-Ser467) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB

Purify method:Unpurification

Species:Human,Mouse,Rat

Gene Name:smad2

Synonyms:JV18-1 MADH2 MADR2 Mad-related protein 2Mothers against DPP homolog 2

Gene Synonyms:

Gene Full Name:

Gene Infomation:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Antigen:Peptide sequence around phosphorylation site of serine 467 (C-S-S-M-S(p)) derived from Human Smad2.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB::1:500-1:1000

Mole Mass:60

Location:

Concentration:1.0mg/mL

Sequence Similarity:

Gene Id:4087

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:The antibody detects endogenous level of Smad2 only when phosphorylated at serine 467.

Storage:

Buffer condition:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.