Anti-Smad3 Mouse mAb (2A1)
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:IHC
Purify method:Affinity purified
Species:Human
Gene Name:SMAD3
Synonyms:hMAD3, HSPC193, LDS1C, MADH3, JV15 2
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:Synthetic peptide conjugated to KLH.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:The Smad3 mouse monoclonal antibody can recognizeendogenous Smad3 proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:2A1-8F2-8B8
Dilution:IHC 1:100-200 (Optimal dilutions should bedetermined by the end user)
Mole Mass:50kDa
Location:Cytoplasm, Nucleus
Concentration:
Sequence Similarity:
Gene Id:4088
SwissProt ID:P84022
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.