Anti-Smad5 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,FC

Purify method:Affinity Chromatography

Species:Human,Rat

Gene Name:

Synonyms:Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5;

Gene Synonyms:

Gene Full Name:

Gene Infomation:DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Antigen:A synthesized peptide derived from human Smad5

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Smad5 Antibody detects endogenous levels of total Smad5

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

Mole Mass:52kDa

Location:Nucleus;

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.

Gene Id:

SwissProt ID:Q99717

Unigene:

Nucleotide Accession:

Tissue specificity:Smad5 Antibody detects endogenous levels of total Smad5

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. Smad5 is a receptor-regulated Smad (R-Smad). Smad5 is required for normal development of the cardiovascular system in vivo; lack of the Smad5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of Smad5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.