Anti-α-Smooth Muscle Actin Mouse mAb (7F4)
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Antibody type:Loading control antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:WB,IHC
Purify method:Affinity purified
Species:Human,Rat,Mouse
Gene Name:ACTA2
Synonyms:AAT6, ACTA2, alpha actin 2, GIG46, ACTSA, ACTVS
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:Synthetic peptide conjugated to KLH.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:The alpha-Smooth Muscle Actin mouse monoclonal antibody candetectendogenous alpha-Smooth Muscle Actin proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:7F4-3C8-1A7
Dilution:WB 1:10,000-100,000 IHC 1:1,000-2,000 (Optimal dilutions should be determined by the end user)
Mole Mass:42kDa
Location:Cytoplasm, Cytoskeleton
Concentration:
Sequence Similarity:
Gene Id:59
SwissProt ID:P62736
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.