Anti-TNF-a Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:ELISA

Purify method:Affinity purified

Species:Human

Gene Name:tnf-a

Synonyms:TNF; DIF; TNFA; TNFSF2; TNF-alpha

Gene Synonyms:APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNF superfamily, member 2; TNF, macrophage deri

Gene Full Name:

Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects TNF-a.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5F3-D10-F11

Dilution:

Mole Mass:

Location:Cell membrane; Single-pass type II membrane protein; Chain Tumor necrosis factor, membrane form : Membrane; Single-pass type II membrane protein Chain Tumor necrosis factor, soluble form : Secreted Chain C-domain 1 : Secreted Chain C-domain 2 : Secreted

Concentration:

Sequence Similarity:Belongs to the tumor necrosis factor family.

Gene Id:7124

SwissProt ID:P01375

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.