Anti-TUBB8 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,IF

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:TUBB8

Synonyms:OOMD; OOMD2; bA631M21.2

Gene Synonyms:

Gene Full Name:tubulin beta 8 class VIII

Gene Infomation:The protein encoded by this gene represents the primary beta-tubulin subunit of oocytes and the early embryo. Defects in this gene, which is primate-specific, are a cause of oocyte maturation defect 2 and infertility. [provided by RefSeq, Mar 2016]

Antigen:Recombinant fusion protein containing a sequence corresponding to amino acids 140-444 of human TUBB8 (NP_817124.1).

Antigen Synonyms:Tubulin beta-8 chain

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB1:500 -1:2000; IHC1:50 -1:100; IF1:50 -1:200(Optimal dilutions should be determined by the end user)

Mole Mass:50kDa

Location:Cytoplasm, Cytoskeleton, Microtubule

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:Q3ZCM7

Unigene:347688

Nucleotide Accession:

Tissue specificity:Expressed at a high level in oocytes, at different stages of development.

Storage:Store at -20°C. Avoid freeze / thaw cycles.

Buffer condition:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Background:

Molar Function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain . TUBB8 has a key role in meiotic spindle assembly and oocyte maturation (PubMed:26789871).

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.