Anti-TriMethyl-Histone H3-K9 Rabbit pAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Methylated
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,IF,IP,ChIP
Purify method:Affinity purified
Species:Human,Mouse,Rat,A wide range of other species
Gene Name:HIST3H3
Synonyms:H3t; H3.4; H3/g; H3FT
Gene Synonyms:
Gene Full Name:histone cluster 3 H3
Gene Infomation:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Antigen:A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Antigen Synonyms:Histone H3.1t
Clonality:Polyclonal antibody
Source:Human
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB1:500 -1:2000; IHC1:50 -1:200; IF1:50 -1:200; IP1:50 -1:200; ChIP1:50 -1:200; CHIPseq1:50 -1:200(Optimal dilutions should be determined by the end user)
Mole Mass:17kDa
Location:Chromosome, Nucleosome core, Nucleus
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:Q16695
Unigene:8290
Nucleotide Accession:
Tissue specificity:Expressed in testicular cells.
Storage:Store at -20°C. Avoid freeze / thaw cycles.
Buffer condition:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background:
Molar Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.