Anti-β-Tubulin Mouse mAb

Antibody type:Loading control antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,ICC/IF

Purify method:Affinity purified

Species:Human,Mouse,Monkey,Goat,Hamster,Rat,Chlamydomonas Reinhardtii

Gene Name:tubb

Synonyms:M40; TUBB1; TUBB5; OK/SW-cl.56

Gene Synonyms:beta 4 tubulin;Beta 5 tubulin;beta Ib tubulin;M40;TUBB1;TUBB5;tubulin beta 1 chain; Tubulin beta 4 chain;tubulin beta 5 chain;Tubulin beta;Tubulin beta chain;tubulin beta polypeptide.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Tubulin beta protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Tubulin beta and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:1E1-E8-H4

Dilution:WB 1:5000,ICC 1:500,IF 1:100

Mole Mass:55kDa

Location:Cytoplasm > cytoskeleton.

Concentration:

Sequence Similarity:Belongs to the tubulin family.

Gene Id:203068

SwissProt ID:P07437

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.

Background:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.