Anti-UBA1 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:GXP1; MGC4781; POC20; GXP1; SMAX2; UBA1; UBE1;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Rho A is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.

Antigen:A synthesized peptide derived from human UBA1

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:UBA1 Antibody detects endogenous levels of total UBA1

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:200 FC 1:20

Mole Mass:118kDa

Location:Cell projection > Lamellipodium;

Concentration:

Sequence Similarity:Belongs to the small GTPase superfamily. Rho family.

Gene Id:

SwissProt ID:P22314

Unigene:

Nucleotide Accession:

Tissue specificity:UBA1 Antibody detects endogenous levels of total UBA1

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.