Anti-UCHL1/PGP9.5 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,ICC

Purify method:Affinity purified

Species:Human,Monkey

Gene Name:uchl1

Synonyms:PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5

Gene Synonyms:UCHL1 / PGP9.5;UCHL1;B220;CD 45;CD45;cd45 antigen;ec3.1.3.48;GP 180;GP180;Human homolog of severe combined immunodeficiency due to PTPRC deficiency;L CA;L-CA;lca;Leukocyte common antigen;LY 5;LY5;Protein tyrosine phosphatase receptor type C;Protein tyrosi

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human UCHL1 / PGP9.5 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of UCHL1 / PGP9.5 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7B8-G5-B8

Dilution:WB 1:1000,ICC 1:300

Mole Mass:25kDa

Location:Cytoplasm

Concentration:

Sequence Similarity:Belongs to the peptidase C12 family.

Gene Id:7345

SwissProt ID:P09936

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol

Background:PGP9.5 is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). Hydrolyze isopeptide bonds between the carboxy-terminal glycine of Ub and the ε-amino group of lysine on target proteins and involved in the cotranslational processing of pro-ubiquitin and ribosomal proteins translated as ubiquitin fusions.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.