Anti-UHRF1(N-term) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human,Mouse

Gene Name:uhrf1

Synonyms:Np95; hNP95; ICBP90; RNF106

Gene Synonyms:Ac2-121;AL022808;E3 ubiquitin-protein ligase UHRF1;EC 6.3.2.-;FLJ21925;Hnp95 Huhrf1; HuNp95;ICBP90;Inverted CCAAT box binding protein of 90 kDa;Inverted CCAAT box binding protein, 90-Kd;Inverted CCAAT box-binding protein of 90 kDa;Liver regeneration-relat

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human UHRF1 protein fragments expressed in E.coil.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of UHRF1 and does not cross-react with related proteins

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:1H9-D3-E11

Dilution:WB 1:1000

Mole Mass:90kDa

Location:Nucleus

Concentration:

Sequence Similarity:Contains 1 PHD-type zinc finger._x000D_Contains 2 RING-type zinc fingers._x000D_Contains 1 ubiquitin-like domain._x000D_Contains 1 YDG domain

Gene Id:29128

SwissProt ID:Q96T88

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol

Background:This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.