Anti-Ubiquitin Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A

Gene Synonyms:

Gene Full Name:

Gene Infomation:Human c-Reactive protein (CRP) was discovered in serum of patients as a nonantibody precipitin of the C-polysaccharide from pneumococci. During acute infection, the amounts of circulating CPR increases 100-1000 fold. Serum CRP values are routinely measured to detect or monitor many human diseases and minor elevations of C-reactive protein are predictive of cardiovascular events in patients with coronary heart disease. CRP promotes host defense, is an efficient activator of the classical C cascade, and shown to be opsonic.The opsonic and C activation activities are responsible for the nonspecific host defense activities providing protection in the preimmune stage of acute infections. Additionally, IL-1 and IL-6 are known to stimulate CRP biosynthesis.

Antigen:A synthesized peptide derived from human Ubiquitin

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Ubiquitin Antibody detects endogenous levels of total Ubiquitin

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

Mole Mass:8kDa

Location:Secreted;

Concentration:

Sequence Similarity:Contains 1 pentaxin domain.

Gene Id:

SwissProt ID:P0CG47

Unigene:

Nucleotide Accession:

Tissue specificity:Ubiquitin Antibody detects endogenous levels of total Ubiquitin

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.